Description: Rabbit polyclonal antibody to VEGFA Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human VEGFA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: VEGFA Alternative Names: VEGF; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPFEntrez Gene (Human): 7422Entrez Gene (Mouse) : 22339Entrez Gene (Rat) : 83785SwissProt (Human): P15692SwissProt (Mouse) : Q00731SwissProt (Rat) : P16612Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of VEGFA expression in Hela (A), A549 (B), mouse liver (C), rat liver (D) whole cell lysates.
• 
  Immunohistochemical analysis of VEGFA staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.