Description: Rabbit polyclonal antibody to Histone H3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000) Gene Symbol: HIST1H3A; HIST1H3; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; H3F3A; H3F3B; H3F3C Alternative Names: HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3; H3F3C; Histone H3.3C; Histone H3.5Entrez Gene (Human): 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968; 126961; 333932; 653604; 3020; 3021; 440093SwissProt (Human): P68431; Q71DI3; P84243; Q6NXT2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H3 expression in NIH3T3 (A) whole cell lysates.
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  Direct ELISA antibody dose-response curve using Anti-Histone H3 (DiMethyl K9) Antibody. Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.