Description: Rabbit polyclonal antibody to p300 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p300. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: EP300 Alternative Names: P300; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300Entrez Gene (Human): 2033Entrez Gene (Mouse) : 328572SwissProt (Human): Q09472SwissProt (Mouse) : B2RWS6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of p300 expression in Hela (A), HEK293T (B) whole cell lysates.
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  Immunohistochemical analysis of p300 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.