Description: Rabbit polyclonal antibody to HMGB1 (Acetyl-K12) Immunogen: KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K12 of human HMGB1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000) Gene Symbol: HMGB1 Alternative Names: HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1Entrez Gene (Human): 3146Entrez Gene (Mouse) : 100862258; 15289Entrez Gene (Rat) : 25459SwissProt (Human): P09429SwissProt (Mouse) : P63158SwissProt (Rat) : P63159Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HMGB1 (Acetyl-K12) expression in DLD (A), rat liver (B) whole cell lysates.
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Direct ELISA antibody dose-response curve using Anti-HMGB1 (Acetyl-K12) Antibody. Antigen (Acetyl-peptide and non-acetyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.