Description: Rabbit polyclonal antibody to USP48 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human USP48. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: USP48 Alternative Names: USP31; Ubiquitin carboxyl-terminal hydrolase 48; Deubiquitinating enzyme 48; Ubiquitin thioesterase 48; Ubiquitin-specific-processing protease 48Entrez Gene (Human): 84196Entrez Gene (Mouse) : 170707Entrez Gene (Rat) : 362636SwissProt (Human): Q86UV5SwissProt (Mouse) : Q3V0C5SwissProt (Rat) : Q76LT8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of USP48 expression in rat muscle (A) whole cell lysates.
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Immunohistochemical analysis of USP48 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.