Description: Rabbit polyclonal antibody to NEK7 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NEK7. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: NEK7 Alternative Names: Serine/threonine-protein kinase Nek7; Never in mitosis A-related kinase 7; NimA-related protein kinase 7Entrez Gene (Human): 140609Entrez Gene (Mouse) : 59125Entrez Gene (Rat) : 360850SwissProt (Human): Q8TDX7SwissProt (Mouse) : Q9ES74SwissProt (Rat) : D3ZBE5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NEK7 expression in HepG2 (A), Jurkat (B), mouse heart (C) whole cell lysates.
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  Immunohistochemical analysis of NEK7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.