Description: Rabbit polyclonal antibody to PRPF6 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PRPF6. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PRPF6 Alternative Names: C20orf14; Pre-mRNA-processing factor 6; Androgen receptor N-terminal domain-transactivating protein 1; ANT-1; PRP6 homolog; U5 snRNP-associated 102 kDa protein; U5-102 kDa proteinEntrez Gene (Human): 24148Entrez Gene (Mouse) : 68879Entrez Gene (Rat) : 366276SwissProt (Human): O94906SwissProt (Mouse) : Q91YR7SwissProt (Rat) : A1A5S1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PRPF6 expression in Hela (A), HEK293T (B), mouse brain (C) whole cell lysates.
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  Immunohistochemical analysis of PRPF6 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.