Description: Rabbit polyclonal antibody to B3GALTL Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human B3GALTL. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: B3GALTL Alternative Names: B3GTL; Beta-1,3-glucosyltransferase; Beta3Glc-T; Beta-3-glycosyltransferase-likeEntrez Gene (Human): 145173SwissProt (Human): Q6Y288Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of B3GALTL expression in HEK293T (A), MCF7 (B), HepG2 (C) whole cell lysates.
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Immunohistochemical analysis of B3GALTL staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.