Description: Rabbit polyclonal antibody to DNA Ligase 1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DNA Ligase 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: LIG1 Alternative Names: DNA ligase 1; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1Entrez Gene (Human): 3978SwissProt (Human): P18858Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DNA Ligase 1 expression in 22RV1 (A), MCF7 (B) whole cell lysates.
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  Immunohistochemical analysis of DNA Ligase 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.