Description: Rabbit polyclonal antibody to PITX1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PITX1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PITX1 Alternative Names: BFT; PTX1; Pituitary homeobox 1; Hindlimb-expressed homeobox protein backfoot; Homeobox protein PITX1; Paired-like homeodomain transcription factor 1Entrez Gene (Human): 5307Entrez Gene (Mouse) : 18740Entrez Gene (Rat) : 113983SwissProt (Human): P78337SwissProt (Mouse) : P70314SwissProt (Rat) : Q99NA7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PITX1 expression in Jurkat (A), NIH3T3 (B) whole cell lysates.
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Immunohistochemical analysis of PITX1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.