Description: Rabbit polyclonal antibody to GRK3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GRK3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ADRBK2 Alternative Names: BARK2; GRK3; Beta-adrenergic receptor kinase 2; Beta-ARK-2; G-protein-coupled receptor kinase 3Entrez Gene (Human): 157Entrez Gene (Mouse) : 320129Entrez Gene (Rat) : 25372SwissProt (Human): P35626SwissProt (Mouse) : Q3UYH7SwissProt (Rat) : P26819Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GRK3 expression in Hela (A), HL60 (B) whole cell lysates.
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  Immunohistochemical analysis of GRK3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.