Description: Rabbit polyclonal antibody to Claudin 3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Claudin 3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CLDN3 Alternative Names: C7orf1; CPETR2; Claudin-3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-receptor 2; Rat ventral prostate.1 protein homolog; hRVP1Entrez Gene (Human): 1365Entrez Gene (Mouse) : 12739Entrez Gene (Rat) : 65130SwissProt (Human): O15551SwissProt (Mouse) : Q9Z0G9SwissProt (Rat) : Q63400Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of Claudin 3 expression in Hela (A), HEK293T (B), NIH3T3 (C) whole cell lysates.
• 
  Immunohistochemical analysis of Claudin 3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.