Description: Rabbit polyclonal antibody to IGFBP3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IGFBP3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: IGFBP3 Alternative Names: IBP3; Insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; IGFBP-3Entrez Gene (Human): 3486Entrez Gene (Mouse) : 16009SwissProt (Human): P17936SwissProt (Mouse) : P47878Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of IGFBP3 expression in HuvEc (A), Raw264.7 (B), mouse brain (C) whole cell lysates.
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  Immunohistochemical analysis of IGFBP3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.