Description: Rabbit polyclonal antibody to PI3K p85 alpha (Phospho-Y607) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y607 of human PI3K p85 alpha protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PIK3R1 Alternative Names: GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alphaEntrez Gene (Human): 5295Entrez Gene (Mouse) : 18708Entrez Gene (Rat) : 25513SwissProt (Human): P27986SwissProt (Mouse) : P26450SwissProt (Rat) : Q63787Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PI3K p85 alpha (Phospho-Y607) expression in Jurkat (A), rat kidney (B) whole cell lysates.
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  Immunohistochemical analysis of PI3K p85 alpha (Phospho-Y607) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.