Description: Rabbit polyclonal antibody to PER2 (Phospho-S662) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S662 of human PER2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PER2 Alternative Names: KIAA0347; Period circadian protein homolog 2; hPER2; Circadian clock protein PERIOD 2Entrez Gene (Human): 8864Entrez Gene (Mouse) : 18627SwissProt (Human): O15055SwissProt (Mouse) : O54943Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PER2 (Phospho-S662) expression in NIH3T3 treated with PMA (A), mouse lung (B) whole cell lysates.
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Immunohistochemical analysis of PER2 (Phospho-S662) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.