Description: Rabbit polyclonal antibody to SHCA (Phospho-Y427) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y427 of human SHCA protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SHC1 Alternative Names: SHC; SHCA; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; SH2 domain protein C1Entrez Gene (Human): 6464Entrez Gene (Mouse) : 20416Entrez Gene (Rat) : 85385SwissProt (Human): P29353SwissProt (Mouse) : P98083SwissProt (Rat) : Q5M824Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SHCA (Phospho-Y427) expression in Hela (A), HEK293T (B) whole cell lysates.
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  Immunohistochemical analysis of SHCA (Phospho-Y427) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.