Description: Rabbit polyclonal antibody to HSP27 (Phospho-S82) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S82 of human HSP27 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: HSPB1 Alternative Names: HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27Entrez Gene (Human): 3315Entrez Gene (Mouse) : 15507SwissProt (Human): P04792SwissProt (Mouse) : P14602Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HSP27 (Phospho-S82) expression in A549 (A), HepG2 (B), K562 (C) whole cell lysates.
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  Immunohistochemical analysis of HSP27 (Phospho-S82) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.