Description: Rabbit polyclonal antibody to IKB alpha (Phospho-Y42) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y42 of human IKB alpha protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: NFKBIA Alternative Names: IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3Entrez Gene (Human): 4792Entrez Gene (Mouse) : 18035Entrez Gene (Rat) : 25493SwissProt (Human): P25963SwissProt (Mouse) : Q9Z1E3SwissProt (Rat) : Q63746Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of IKB alpha (Phospho-Y42) expression in K562 (A), Jurkat IL1b-treated (B), Raw264.7 LPS-treated (C), H9C2 LPS-treated (D) whole cell lysates.
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  Immunohistochemical analysis of IKB alpha (Phospho-Y42) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.