Description: Rabbit polyclonal antibody to RB1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RB1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: RB1 Alternative Names: Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110Entrez Gene (Human): 5925Entrez Gene (Mouse) : 19645Entrez Gene (Rat) : 24708SwissProt (Human): P06400SwissProt (Mouse) : P13405SwissProt (Rat) : P33568Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RB1 expression in A549 (A), NIH3T3 (B), K562 (C), PC12 (D) whole cell lysates.
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  Immunohistochemical analysis of RB1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.