Description: Rabbit polyclonal antibody to PLC gamma 1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PLC gamma 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PLCG1 Alternative Names: PLC1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1; PLC-gamma-1Entrez Gene (Human): 5335Entrez Gene (Mouse) : 18803Entrez Gene (Rat) : 25738SwissProt (Human): P19174SwissProt (Mouse) : Q62077SwissProt (Rat) : P10686Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PLC gamma 1 expression in Hela (A), HepG2 (B), mouse brain (C), rat kidney (D) whole cell lysates.
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Immunohistochemical analysis of PLC gamma 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.