Description: Rabbit polyclonal antibody to ATF2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATF2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100) Gene Symbol: ATF2 Alternative Names: CREB2; CREBP1; Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; HB16; Histone acetyltransferase ATF2; cAMP response element-binding protein CRE-BP1Entrez Gene (Human): 1386Entrez Gene (Mouse) : 102641666; 11909Entrez Gene (Rat) : 81647SwissProt (Human): P15336SwissProt (Mouse) : P16951SwissProt (Rat) : Q00969Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ATF2 expression in Hela (A), MCF7 (B), SP20 (C), rat brain (D) whole cell lysates.
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  Immunohistochemical analysis of ATF2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.