Description: Rabbit polyclonal antibody to c-Jun Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human c-Jun. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: JUN Alternative Names: Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39Entrez Gene (Human): 3725Entrez Gene (Mouse) : 16476Entrez Gene (Rat) : 24516SwissProt (Human): P05412SwissProt (Mouse) : P05627SwissProt (Rat) : P17325Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of c-Jun expression in Hela (A), mouse kidney (B), rat brain (C) whole cell lysates.
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  Immunohistochemical analysis of c-Jun staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.