Description: Rabbit polyclonal antibody to FANCD2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human FANCD2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: FANCD2 Alternative Names: FACD; Fanconi anemia group D2 protein; Protein FACD2Entrez Gene (Human): 2177Entrez Gene (Mouse) : 211651Entrez Gene (Rat) : 312641SwissProt (Human): Q9BXW9SwissProt (Mouse) : Q80V62SwissProt (Rat) : Q6IV68Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of FANCD2 expression in HT29 (A), SP20 (B), mouse liver (C) whole cell lysates.
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Immunohistochemical analysis of FANCD2 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.