Description: Rabbit polyclonal antibody to PKC delta (Phospho-S645) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S645 of human PKC delta protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PRKCD Alternative Names: Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-deltaEntrez Gene (Human): 5580SwissProt (Human): Q05655Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PKC delta (Phospho-S645) expression in A549 (A), MCF7 (B) whole cell lysates.
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  Immunohistochemical analysis of PKC delta (Phospho-S645) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.