Description: Rabbit polyclonal antibody to CPI17 (Phospho-T38) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T38 of human CPI17 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PPP1R14A Alternative Names: CPI17; PPP1INL; Protein phosphatase 1 regulatory subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17Entrez Gene (Human): 94274Entrez Gene (Mouse) : 68458Entrez Gene (Rat) : 114004SwissProt (Human): Q96A00SwissProt (Mouse) : Q91VC7SwissProt (Rat) : Q99MC0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CPI17 (Phospho-T38) expression in SHSY5Y (A), Raw264.7 (B) whole cell lysates.
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  Immunohistochemical analysis of CPI17 (Phospho-T38) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Direct ELISA antibody dose-response curve using Anti-CPI17 (Phospho-T38) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.