Description: Rabbit polyclonal antibody to HSD3B7 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSD3B7. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: HSD3B7 Alternative Names: 3 beta-hydroxysteroid dehydrogenase type 7; 3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase; C(27) 3-beta-HSD; Cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenaseEntrez Gene (Human): 80270Entrez Gene (Mouse) : 101502Entrez Gene (Rat) : 246211SwissProt (Human): Q9H2F3SwissProt (Mouse) : Q9EQC1SwissProt (Rat) : O35048Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HSD3B7 expression in Lovo (A), MCF7 (B), Hela (C) whole cell lysates.
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  Immunohistochemical analysis of HSD3B7 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.