Description: Rabbit polyclonal antibody to UBF Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human UBF. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: UBTF Alternative Names: UBF; UBF1; Nucleolar transcription factor 1; Autoantigen NOR-90; Upstream-binding factor 1; UBF-1Entrez Gene (Human): 7343Entrez Gene (Rat) : 25574SwissProt (Human): P17480SwissProt (Mouse) : P25976SwissProt (Rat) : P25977Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of UBF expression in Jurkat (A), rat muscle (B) whole cell lysates.
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  Immunohistochemical analysis of UBF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.