Description: Rabbit polyclonal antibody to PIAS1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human PIAS1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PIAS1 Alternative Names: DDXBP1; E3 SUMO-protein ligase PIAS1; DEAD/H box-binding protein 1; Gu-binding protein; GBP; Protein inhibitor of activated STAT protein 1; RNA helicase II-binding proteinEntrez Gene (Human): 8554Entrez Gene (Mouse) : 56469SwissProt (Human): O75925SwissProt (Mouse) : O88907Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PIAS1 expression in HuvEc (A), Jurkat (B) whole cell lysates.
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  Immunohistochemical analysis of PIAS1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.