Description: Rabbit polyclonal antibody to CHK1 (Phospho-S301) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S301 of human CHK1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: CHEK1 Alternative Names: CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1Entrez Gene (Human): 1111Entrez Gene (Mouse) : 12649Entrez Gene (Rat) : 140583SwissProt (Human): O14757SwissProt (Mouse) : O35280SwissProt (Rat) : Q91ZN7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CHK1 (Phospho-S301) expression in HEK293T (A) whole cell lysates.
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Immunofluorescent analysis of CHK1 (Phospho-S301) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.