Description: Rabbit polyclonal antibody to JUND Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human JUND. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100), EMSA (Use at an assay dependent concentration) Gene Symbol: JUND Alternative Names: Transcription factor jun-DEntrez Gene (Human): 3727Entrez Gene (Mouse) : 16478Entrez Gene (Rat) : 24518SwissProt (Human): P17535SwissProt (Mouse) : P15066SwissProt (Rat) : P52909Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of JUND expression in A549 (A), Jurkat (B) whole cell lysates.
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Immunohistochemical analysis of JUND staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.