Description: Rabbit polyclonal antibody to Dynamin 1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Dynamin 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: DNM1 Alternative Names: DNM; Dynamin-1Entrez Gene (Human): 1759Entrez Gene (Mouse) : 13429Entrez Gene (Rat) : 140694SwissProt (Human): Q05193SwissProt (Mouse) : P39053SwissProt (Rat) : P21575Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Dynamin 1 expression in NIH3T3 (A), mouse brain (B), PC12 (C) whole cell lysates.
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Immunohistochemical analysis of Dynamin 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.