Description: Rabbit polyclonal antibody to MID1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human MID1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: MID1 Alternative Names: FXY; RNF59; TRIM18; XPRF; E3 ubiquitin-protein ligase Midline-1; Midin; Putative transcription factor XPRF; RING finger protein 59; RING finger protein Midline-1; Tripartite motif-containing protein 18Entrez Gene (Human): 4281Entrez Gene (Mouse) : 17318Entrez Gene (Rat) : 54252SwissProt (Human): O15344SwissProt (Mouse) : O70583SwissProt (Rat) : P82458Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MID1 expression in HEK293T (A) whole cell lysates.
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  Immunohistochemical analysis of MID1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of MID1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).