Description: Rabbit polyclonal antibody to CDC37 (pS13) Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CDC37 (pS13). The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CDC37 Alternative Names: CDC37A; Hsp90 co-chaperone Cdc37; Hsp90 chaperone protein kinase-targeting subunit; p50Cdc37Entrez Gene (Human): 11140Entrez Gene (Mouse) : 12539Entrez Gene (Rat) : 114562SwissProt (Human): Q16543SwissProt (Mouse) : Q61081SwissProt (Rat) : Q63692Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDC37 (pS13) expression in HuvEc (A), K562 (B), NIH3T3 (C) whole cell lysates.
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Immunohistochemical analysis of CDC37 (Phospho-S13) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.