Description: Rabbit polyclonal antibody to PKC theta (Phospho-S695) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S695 of human PKC theta protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PRKCQ Alternative Names: PRKCT; Protein kinase C theta type; nPKC-thetaEntrez Gene (Human): 5588SwissProt (Human): Q04759Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PKC theta (Phospho-S695) expression in Jurkat PMA-treated (A), HEK293T PMA-treated (B) whole cell lysates.
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  Immunohistochemical analysis of PKC theta (Phospho-S695) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.