Description: Rabbit polyclonal antibody to Cyclin D3 (Phospho-T283) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T283 of human Cyclin D3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CCND3 Alternative Names: G1/S-specific cyclin-D3Entrez Gene (Human): 896Entrez Gene (Mouse) : 12445Entrez Gene (Rat) : 25193SwissProt (Human): P30281SwissProt (Mouse) : P30282SwissProt (Rat) : P48961Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Cyclin D3 (Phospho-T283) expression in HepG2 UV-treated (A), K562 UV-treated (B) whole cell lysates.
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  Immunohistochemical analysis of Cyclin D3 (Phospho-T283) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.