Description: Rabbit polyclonal antibody to PDGFB Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PDGFB. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PDGFB Alternative Names: PDGF2; SIS; Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; BecaplerminEntrez Gene (Human): 5155Entrez Gene (Mouse) : 18591Entrez Gene (Rat) : 24628SwissProt (Human): P01127SwissProt (Mouse) : P31240SwissProt (Rat) : Q05028Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PDGFB expression in U2OS (A), mouse liver (B), mouse muscle (C), rat liver (D), rat muscle (E) whole cell lysates.
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Immunohistochemical analysis of PDGFB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.