Description: Rabbit polyclonal antibody to Histone H4 (DiMethyl-K20) Immunogen: KLH-conjugated synthetic Di-methylated peptide corresponding to residues surrounding K20 of human Histone H4 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000) Gene Symbol: HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST4H4 Alternative Names: H4/A; H4FA; H4/I; H4FI; H4/G; H4FG; H4/B; H4FB; H4/J; H4FJ; H4/C; H4FC; H4/H; H4FH; H4/M; H4FM; H4/E; H4FE; H4/D; H4FD; H4/K; H4FK; H4/N; H4F2; H4FN; HIST2H4; H4/O; H4FO; Histone H4Entrez Gene (Human): 121504; 554313; 8294; 8359; 8360; 8361; 8362; 8363; 8364; 8365; 8366; 8367; 8368; 8370Entrez Gene (Mouse) : 100041230; 102641229; 319155; 319156; 319157; 319158; 319159; 319160; 319161; 320332; 326619; 326620; 69386; 97122Entrez Gene (Rat) : 100360950; 100912290; 100912418; 100912564; 102548682; 102551184; 102557184; 291152; 295277; 500351; 502913; 64627; 680097SwissProt (Human): P62805SwissProt (Mouse) : P62806SwissProt (Rat) : P62804Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Histone H4 (DiMethyl-K20) expression in mouse heart (A), rat kidney (B) whole cell lysates.
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Direct ELISA antibody dose-response curve using Anti-Histone H4 (DiMethyl-K20) Antibody. Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.