Description: Rabbit polyclonal antibody to ECM1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ECM1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ECM1 Alternative Names: Extracellular matrix protein 1; Secretory component p85Entrez Gene (Human): 1893Entrez Gene (Mouse) : 13601SwissProt (Human): Q16610SwissProt (Mouse) : Q61508Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ECM1 expression in MCF7 (A), A375 (B), mouse brain (C) whole cell lysates.
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  Immunohistochemical analysis of ECM1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.