Description: Rabbit polyclonal antibody to RFPL4B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RFPL4B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: RFPL4B Alternative Names: RNF211; Ret finger protein-like 4B; RING finger protein 211Entrez Gene (Human): 442247SwissProt (Human): Q6ZWI9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RFPL4B expression in SKOVCAR3 (A), K562 (B) whole cell lysates.
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Immunohistochemical analysis of RFPL4B staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.