Description: Rabbit polyclonal antibody to FRS2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human FRS2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: FRS2 Alternative Names: Fibroblast growth factor receptor substrate 2; FGFR substrate 2; FGFR-signaling adaptor SNT; Suc1-associated neurotrophic factor target 1; SNT-1Entrez Gene (Human): 10818Entrez Gene (Mouse) : 327826SwissProt (Human): Q8WU20SwissProt (Mouse) : Q8C180Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of FRS2 expression in U87MG (A), H9C2 (B) whole cell lysates.
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  Immunohistochemical analysis of FRS2 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.