Description: Rabbit polyclonal antibody to S6K1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human S6K1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: RPS6KB1 Alternative Names: STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KAEntrez Gene (Human): 6198Entrez Gene (Mouse) : 72508Entrez Gene (Rat) : 83840SwissProt (Human): P23443SwissProt (Mouse) : Q8BSK8SwissProt (Rat) : P67999Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of S6K1 expression in Hela (A), rat oviduct (B), 3T3L1 (C), PC12 (D) whole cell lysates.
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Immunohistochemical analysis of S6K1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.