Description: Rabbit polyclonal antibody to DARPP32 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DARPP32. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PPP1R1B Alternative Names: DARPP32; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoproteinEntrez Gene (Human): 84152Entrez Gene (Mouse) : 19049Entrez Gene (Rat) : 360616SwissProt (Human): Q9UD71SwissProt (Mouse) : Q60829SwissProt (Rat) : Q6J4I0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DARPP32 expression in mouse heart (A), rat heart (B) whole cell lysates.
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  Immunohistochemical analysis of DARPP32 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.