Description: Rabbit polyclonal antibody to CENPA (Phospho-S7) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S7 of human CENPA protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: CENPA Alternative Names: Histone H3-like centromeric protein A; Centromere autoantigen A; Centromere protein A; CENP-AEntrez Gene (Human): 1058SwissProt (Human): P49450Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of CENPA (Phospho-S7) expression in HEK293T (A), Hela (B), DLD (C), rat testis (D), rat spleen (E) whole cell lysates.
• 
  Immunofluorescent analysis of CENPA (Phospho-S7) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
• 
  Direct ELISA antibody dose-response curve using Anti-CENPA (Phospho-S7) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.