Description: Rabbit polyclonal antibody to Aquaporin 2 (Phospho-S256) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S256 of human Aquaporin 2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: AQP2 Alternative Names: Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting ductEntrez Gene (Human): 359Entrez Gene (Mouse) : 11827Entrez Gene (Rat) : 25386SwissProt (Human): P41181SwissProt (Mouse) : P56402SwissProt (Rat) : P34080Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Aquaporin 2 (Phospho-S256) expression in mouse kidney (A), rat kidney (B), mouse lung (C) whole cell lysates.
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  Immunohistochemical analysis of Aquaporin 2 (Phospho-S256) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.