Description: Rabbit polyclonal antibody to PLA2G4D Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PLA2G4D. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PLA2G4D Alternative Names: Cytosolic phospholipase A2 delta; cPLA2-delta; Phospholipase A2 group IVDEntrez Gene (Human): 283748SwissProt (Human): Q86XP0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PLA2G4D expression in Hela (A) whole cell lysates.
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Immunohistochemical analysis of PLA2G4D staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.