Description: Rabbit polyclonal antibody to Laminin alpha 3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Laminin alpha 3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: LAMA3 Alternative Names: LAMNA; Laminin subunit alpha-3; Epiligrin 170 kDa subunit; E170; Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alphaEntrez Gene (Human): 3909Entrez Gene (Mouse) : 16774SwissProt (Human): Q16787SwissProt (Mouse) : Q61789Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Laminin alpha 3 expression in AML12 (A), HEK293T (B), SGC7901 (C) whole cell lysates.
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Immunofluorescent analysis of Laminin alpha 3 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.