Description: Rabbit polyclonal antibody to Collagen 16 alpha 1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Collagen 16 alpha 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: COL16A1 Alternative Names: Collagen alpha-1(XVI) chainEntrez Gene (Human): 1307SwissProt (Human): Q07092Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Collagen 16 alpha 1 expression in A375 (A) whole cell lysates.
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Immunohistochemical analysis of Collagen 16 alpha 1 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.