Description: Rabbit polyclonal antibody to GAB2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GAB2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: GAB2 Alternative Names: KIAA0571; GRB2-associated-binding protein 2; GRB2-associated binder 2; Growth factor receptor bound protein 2-associated protein 2; pp100Entrez Gene (Human): 9846SwissProt (Human): Q9UQC2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GAB2 expression in K562 (A), MCF7 (B), H1792 (C), A549 (D) whole cell lysates.
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Immunohistochemical analysis of GAB2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.