Description: Rabbit polyclonal antibody to CD144 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD144. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CDH5 Alternative Names: Cadherin-5; 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD144Entrez Gene (Human): 1003Entrez Gene (Mouse) : 12562SwissProt (Human): P33151SwissProt (Mouse) : P55284Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD144 expression in A375 (A), U87MG (B), mouse brain (C), rat brain (D) whole cell lysates.
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  Immunohistochemical analysis of CD144 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.