Description: Rabbit polyclonal antibody to CDC25 (Phospho-S916) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S916 of human CDC25 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: RASGRF1 Alternative Names: RASGRF1; CDC25; GNRP; GRF1; Ras-specific guanine nucleotide-releasing factor 1; Ras-GRF1; Guanine nucleotide-releasing protein; GNRP; Ras-specific nucleotide exchange factor CDC25Entrez Gene (Human): 5923Entrez Gene (Mouse) : 19417SwissProt (Human): Q13972SwissProt (Mouse) : P27671Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CDC25 (Phospho-S916) expression in U87MG (A), Panc1 (B), A549 (C) whole cell lysates.
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  Immunohistochemical analysis of CDC25 (Phospho-S916) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of CDC25 (Phospho-S916) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.